Joshua Ramsey, Ph.D.

Associate Professor
Chemical Engineering

Education

B.S. Chemical Engineering, Oklahoma State University, 2000
M.S. Chemical Engineering, University of Illinois, 2003
Ph.D. Chemical  & Biomolecular Engineering, University of Illinois, 2006PhRMA Foundation Fellow, University of Kansas, 2007

Research Interests

Engineering Novel Gene Delivery Vectors

Safe and efficient delivery of genetic material remains a serious challenge limiting the field of gene therapy.  Our group is interested in developing novel methods for gene delivery.  We are currently funded by the American Heart Association to investigate hybrid viral/synthetic gene delivery vectors to target vascular tissue and treat critical limb and myocardial ischemia.

Improving Industrial Relevant Enzymes and Microorganisms

Directed evolution is a technique that has been used to successfully re-engineer enzymes for a variety of industrial applications.  Such enzymes show enhanced or novel substrate activity, improved stability in organic solvents, or greater thermostability.  Our lab is interested in using this technique to produce novel enzymes and microorganisms that meet the growing demand for industrially relevant enzymes and microorganisms.

We are currently funded by the Oklahoma Transportation Center to investigate microbial degradation of concrete infrastructure.  The microbes that we are studying are believed to produce an acid that attacks concrete and may shorten the lifespan of our concrete infrastructure.

Major Areas of Research

Engineering Novel Gene Delivery Vectors
Improving Industrial Relevant Enzymes and Microorganisms
Microbial Deterioration of Concrete Infrastructure
Virtual Design and Screening of Therapeutic Compounds

Key Publications

S. Vupputuri, S. Karode, B.J. Neely and J.D. Ramsey, “Protein Impurities from Cell Culture Dramatically Impact Transduction Efficiency of Polymer/Virus Hybrid Vectors.” Submitted to Journal of Biotechnology, 2012.

K. Singarapu, I. Pal, and J.D. Ramsey, “Modified Polyethylenimine Used to Enhance Adenovirus Gene Delivery.” Submitted to Drug Discoveries and Therapeutics, 2012.

I. Pal, M. Mozafari, K. Singarapu, L. Tayebi, and J.D. Ramsey, “Therapeutic efficacy of adenovirus in combination with polyethylene glycol grafted to poly-L-lysine for gene delivery: Gene expression, cytocompatibility and hemocompatibility.” Submitted to Biomaterials, 2012.

A. Nigatu and J.D. Ramsey, “Development of an Improved Adenovirus Gene Therapy Vector That Employs PEG and Cell Penetrating Peptides for Transformation of Difficult to Infect Cell Lines.” Manuscript in preparation.

S. Vupputuri, P. Rajakaruna, T. Ley, G. Wilber, B. Fathepure, J.D. Ramsey. “Characterization of an Acid-Producing Microorganism Collected from a Deteriorating Bridge Site.” Manuscript in preparation.

Pal, I. and J.D. Ramsey, “The Role of the Lymphatic System in Vaccine Trafficking and Immune Response.”Advanced Drug Delivery Reviews, in press, 2011.